Investigation of the Effects of Propolis on Some Blood Parameters in Rainbow Trout (Oncorhynchus Mykiss) Exposed to Long-Term Bısphenol-A

Abstract

Introduction: In recent years, studies have revealed the harmful effects of Bisphenol-A (BPA), whose use and production have increased, on living organisms. Many chemicals, like BPA, designed to simplify life, adversely affect it. Therefore, it is needed to identify alternative substances to replace these chemicals or to develop new compounds that can mitigate their harmful effects. Research has shown that many food products with antioxidant properties can counteract the toxic effects of xenobiotics. One such product is propolis, which has been used in alternative medicine for many years. It aims to investigate the effects of BPA long term sexposure on certain blood parameters in rainbow trout. Additionally, it seeks to explore the potential protective role of propolis in alleviating these harmful effects.

Material-Method: Rainbow trout were used as the experimental material in this study. The experimental setup consisted of a total of 8 groups, including fish exposed to BPA concentrations of 500, 1000, and 1500 μg/L, as well as BPA + propolis groups, where fish were fed with propolis-supplemented feed during the exposure. It lasted for 7 weeks. After experiment, blood samples were collected following the dissection of the fish, centrifuged, and analyzed for the necessary blood parameters.

Results: It was observed that different doses of BPA and propolis applied to rainbow trout affected the values of certain blood parameters following chronic toxicity exposure. The blood values obtained from the fish were analyzed and statistically evaluated.

Conclusion and Discussion: After analyses, different doses of BPA increased the levels of certain blood parameters in fish, indicating toxic effects. Other groups treated with propolis, a natural antioxidant, showed reduced levels of these blood parameters. Consequently, it can be stated that the application of propolis mitigated the toxicity caused by BPA in rainbow trout.