Ameliorative Effects of Citrus limetta Raw Juice on Cyclophosphamide-Induced Organ Toxicity in Rats

Özet

The administration of cyclophosphamide (CYP) as a chemotherapeutic drug has been associated with organ toxicities by generative oxidative stress through its reactive metabolites. Thus, current study is aimed to explore the ameliorative effect of Citrus limetta (CL) raw juice treatment against CYP-induced nephrotoxicity and hepatotoxicity in rats. Following one week acclimatization, female Albino rats (N=24), were equally and randomly divided into four groups: control negative (CN) provided with normal feed and water intake, positive control group (CYP) was given intra-peritoneal injection of CYP at 20mg/kg/bw, positive control group (CL) was given oral CL treatment at 25ml/kg/bw without toxicity induction by CYP and treatment group (CYP+CL) received CL juice (25mL/Kg BW orally) after injection of CYP (20mg/kg intra-peritoneal). After 7 days of treatment, blood and tissue samples were collected and processed for biochemical markers and histopathology of liver and renal tissue. After statistical analyses, our results showed that CL juice significantly (p≤0.05) restored hepatic serum markers (ALT, AST, and total proteins), renal profile (creatinine, BUN, and uric acid), and cell injury markers (LDH and CK). Histopathological findings further supported biochemical findings and confirmed that CL juice preserved the architectural integrity and restored functions of the liver and kidney. Based on our findings, it is implied that CL may offer hepatic and renal protection by ameliorating oxidative stress produced by  CYP administration.