Isolation of Cellulolytic Bacterium from the Digestive Tract of the Leptinotarsa decemlineata (Colorado Potato Beetle)

Özet

The objective of this study was to isolate and identify cellulose-degrading bacteria from the adult digestive tract of the Leptinotarsa decemlineata (Colorado potato beetle). Soluble carboxymethyl cellulose (CMC) degradation assays were used to identify five bacterial strains and assess their cellulolytic activity. The development of a clear zone on CMC plate media and the cellulolytic index were used to calculate cellulolytic activity. The highest cellulolytic index (2.2) was found in MOC6. This strain was identified and described using morphological, biochemical, and partial 16S rDNA sequencing. New cellulose-degrading bacteria from the Leptinotarsa decemlineata's gut have been identified in this investigation. The isolate had relatively high cellulolytic activity was identified as Bacillus pumilus strain MOC6. The potential use of this strain in the breakdown of cellulose makes its identification significant.